This stage is very long and fairly wavy at first, running through the hills around Siena, and also crossing Siena. To be noted, there are four consecutive level crossings on the same (secondary) line in the first part. After Torrita di Siena, the gradients ease out and the route continues mostly on straight roads, narrowed at points, along the plain surrounding Lake Trasimeno that leads to Perugia. Then comes the long, manageable climb of Casacastalda, whose descent leads directly to the finish. The road is very bumpy and demanding, especially on the descent.
The last kilometres are undulating and mostly downhill until the last kilometre, where a rather sharp turn to the left marks the beginning of the finale. The road first presents wide bends and then ends in a straight line. The final straight has a slight uphill gradient of around 4%. The finish line sits on an 8 m wide asphalt straight.